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. 2014 Nov 14;9(11):e112878. doi: 10.1371/journal.pone.0112878

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© 2014 Fan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A presents a comparison of the expression of Dusp5 mRNA in cerebral arteries of FHH versus FHH.1^BN rats. The upper portion of the figure presents the representative images of gels showing the qPCR products and the bar graph below compares the expression levels. Panel B presents a comparison of the expression of phosphorylated-ERK1/2, total ERK1/2, phosphorylated-PKC and beta-Actin in the brain of FHH and FHH.1^BN rats. The upper panel presents the representative images and the lower panel presents the relative quantitation. Panel C presents a comparison of expression of these proteins in cerebral microvessels of FHH as compared with FHH.1^BN rats. All of the vessels isolated from one strain were pooled into a single sample. The upper panel presents the representative images and the lower panel presents the quantitation of the images. Mean values ± SE from 3 rats per strain are presented in Panel A and Panel B. Panel C represents the results from duplicate aliquots run from a single pooled microvessel sample isolated from 8–10 rats per strain. * indicates a significant difference from the corresponding value in FHH rats.