Figure 5. Comparison of the expression and activity of Dusp5 in WBCs isolated from Dusp5 ZFN KO versus FHH.1^BN rats.

Panel A: The expression of Dusp5 protein in WBCs is nearly absent in Dusp5 KO versus FHH.1^BN rats. The levels of phosphorylated-ERK1/2 protein are significantly increased in Dusp5 KO compared to FHH.1^BN rats, but there is no change in the expression of total ERK or beta-Actin. Panel B presents the results of the expression of Dusp5, p-ERK1/2, total ERK1/2 and β-Actin protein from duplicate aliquots of a single pooled microvessel sample isolated from 8–10 Dusp5 KO and FHH.1^BN rats. The upper panel presents a representative image of the gels and the lower panel presents the quantitation of the images above. Mean values ± SE from 3 rats per strain are presented in Panel A. * indicates a significant difference from the corresponding value in Dusp5 KO rats.