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. 2014 Nov 14;9(11):e112647. doi: 10.1371/journal.pone.0112647

Table 3. The Percentages of Y93 Wild and Y93H Mutant Oligonucleosides Measured by Real-Time PCR Using Cycling Probe Mixtures Following Adjustment to Different Percent Volumes of Both Oligonucleosides.

Sample Copies of oligonucleosides evaluated by the real-time PCR Predicted percentages (%)
Y93 wild Y93H mutant Calculated percentages of Y93H mutant (%)
Copies Log Copies Log
A 1.13×106 6.05 0 0 0.0 0
B 1.80×105 5.26 4.16×103 3.64 2.2 5
C 1.05×106 6.02 4.35×104 4.64 4.0 10
D 1.02×106 6.01 3.31×105 5.52 24.4 30
E 7.89×105 5.90 6.16×105 5.79 43.7 50
F 3.63×105 5.56 9.12×105 5.96 71.5 70
G 3.39×104 4.53 1.26×106 6.10 97.4 90
H 0 0 9.12×105 5.96 100.0 100