Table 3. The Percentages of Y93 Wild and Y93H Mutant Oligonucleosides Measured by Real-Time PCR Using Cycling Probe Mixtures Following Adjustment to Different Percent Volumes of Both Oligonucleosides.
Sample | Copies of oligonucleosides evaluated by the real-time PCR | Predicted percentages (%) | ||||
Y93 wild | Y93H mutant | Calculated percentages of Y93H mutant (%) | ||||
Copies | Log | Copies | Log | |||
A | 1.13×106 | 6.05 | 0 | 0 | 0.0 | 0 |
B | 1.80×105 | 5.26 | 4.16×103 | 3.64 | 2.2 | 5 |
C | 1.05×106 | 6.02 | 4.35×104 | 4.64 | 4.0 | 10 |
D | 1.02×106 | 6.01 | 3.31×105 | 5.52 | 24.4 | 30 |
E | 7.89×105 | 5.90 | 6.16×105 | 5.79 | 43.7 | 50 |
F | 3.63×105 | 5.56 | 9.12×105 | 5.96 | 71.5 | 70 |
G | 3.39×104 | 4.53 | 1.26×106 | 6.10 | 97.4 | 90 |
H | 0 | 0 | 9.12×105 | 5.96 | 100.0 | 100 |