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The chicken HS4 insulator behaves as a matrix attachment site in vitro. An in vitro MAR assay by using a 32P-labeled 1.2-kb HS4 insulator probe (Ins) or a control λ DNA probe. Prepared matrices (lithium salt and 2 M NaCl) were incubated with the labeled DNA fragments and washed, and bound DNA was precipitated and resolved by agarose gel electrophoresis. The 1.2-kb insulator specifically bound, whereas the control DNA did not.
