Figure 3.
Polypeptides display diminished membrane activity and cytotoxicity upon UV treatment. (A) Uptake of FITC-Tris in HeLa and COS-7 cells in the presence of P20 and UV-treated P20 (n=3). (B) Cytotoxicity of polypeptide/DNA complexes in HeLa cells (N/P ratio of 20, 5 μg DNA/mL) with/without in situ UV treatment (20 mW/cm2, 5 min) as assessed by the MTT assay (n=3); ns denotes no significant difference (p>0.05).