Table 1.

Key molecules potentially responsible for K369R’s tumor promoting function and K369R-regulated genes that identified these key molecules in MetaCore analysis.

Key molecules	Genes regulated by K369R in DU 145 tumor
From the comparison between K369R and the PLHCX vector control
RELA (p65)	Upregulated: JUN, C3, CD44, CAV1, CFB, GNAI2, LGALS1, KLF6, LAMB3, OPTN, PKM, PRKCDBP, RIPK2, PSAP, SMS, TGM2, TNFAIP2, CASP4, ERAP2, MET, SERPINE1, TNC; Downregulated: STAMBPL1, EPAS1, EHF, FBLN1, GLRX, IGFBP2, IGFBP3, ICAM1, NFKBIA, PAG1, GDF15, TFPI2, VEGFA, ZFP36
p53	Upregulated: ACTA2, SERPINA1, CRYAB, DDIT3, TNFSF9, COL4A1, CAV1, CCDC85B, FHL2, GADD45A, GPX1, RNF128, HSPB1, ITGB4, CKM, LAMC2, PMAIP1, P4HA2, PERP, PHLDA3, PLK2, PRNP, RRAD, RHOC, S100A2, TNC, THBS1, AXL, VIM; Downregulated: ANXA4, CPSF6, CHEK2, DAPK1, DKK1, EDIL3, EPCAM, IGFBP2, IGFBP3, LIF, DUSP4, NDRG1, GDF15, PTP4A1, RGCC, SLC6A6, STMN1, TFPI2, TOP2A, TXNIP, TMEM205, VEGFA, ZFP36, PLAGL1
CREB1	Upregulated: VGF, IL-32, RRAD, ETS2, NOXA, Cyr61, SMRT, PAI-1; Downregulated: ASS1, BTG1, CEBPD, CGA, CHMP1B, GJA1, ICA1, LAPTM4B, LIF, LTBP1, NR4A3, NR4A1, PDE4D, GDF15, PER1, PTP4A1, STMN1, TOB1, VEGFA.
MYC	Upregulated: JUN, BMI1, CD44, CLIC4, S100A11, DSG2, ETS2, ETV6, FGFR1, FSTL3, GNAI2, GADD45A, GALNT2, LGALS1, HERPUD1, KLF6, MYO1B, MYO1C, PMAIP1, OPTN, CDH3, PEA15, ATP2B4, PMEPA1, RALB, THBS1; Downregulated: ABCA2, ASS1, ADM, BASP1, BTG1, CEBPD, CEACAM5, CHEK2, DKK1, DNAJC12, ICAM1, PRPS2, NME4, NDRG1, NOL6, NUB1, NR4A1, PTP4A1, PEG10, JUP, VEGFA, WIF1, ZFP36
JUN	Upregulated: CD44, CLIC4, CYR61, GADD45A, MET, IL18, IL32, SPARC, SERPINE1, TSPO, PSAP, ST5, TNC, VIM; Downregulated: CEBPD, DKK1, NFKBIA, BPIFA1, SLC6A6, VEGFA, CYBA, ALDH2, CGA, NR4A1, SFTPB, F3
From the comparison between K369R and KLF5
ER	Upregulated: JUN, ANXA3, TGFBI, CD44, CAV1, HES1, HSPB1, PMAIP1, SAT1, TNS4, DDIT3, C6orf141, CASP4, DCBLD2, GAS6, KRT19, KRT7, CLIC1, CDH3, PLAUR, PLOD2, PITX1, TGM2, TMEM2; Downregulated: APOE, ASS1, ATP11A, AQP3, CGA, SLC9A3R1, AGR2, IGFBP2, IGFBP3, MALL, ERRFI1, MUC1, NELL2, NR4A1, PDK4, SLC26A2, SLC6A6, F3, XBP1, FOS
AR	Upregulated: KRT7, ADAM9, AMIGO2, JUN, CD44, CAV1, CYR61, GAS6, FBXO32, PMAIP1, CDH3, PMEPA1, TNS4; Downregulated: SLPI, CITED2, AGR2, IGFBP3, ITGB5, MUC1, NDRG1, PER1, PEG10, SLC26A2, STOM, TM4SF1, F3, XBP1, ZNF189
SP1	Upregulated: JUN, SERPINA1, BMI1, BNC1, DDIT3, COL4A1, COL4A2, S100A6, CAV1, F13A1, FGFR1, FHL2, GFPT2, MET, HSPB1, HSPA1A, KRT19, KRT7, LAMC2, MICB, MYH9, SPARC, SERPINE1, PKM, PLAU, PLAUR, SAT1, TGM2, TIMP2, TNC, TNNT3, AXL, VIM, XAGE1B; Downregulated: AKAP12, AKR1C2, AKR1C1, APOE, ASS1, CEBPD, CEACAM6, CITED2, CRABP2, CEA, CGA, CCND3, EPAS1, EPCAM, GSTA4, IGFBP2, IGFBP3, ICAM1, IDH2, ITPR1, ITGB5, LAMA1, MDM2, KITLG, MUC1, NR4A1, GDF15, PDK4, PGD, SMARCA1, SFTPB, TFPI2, F3, UGDH, FOS
CREB1	Upregulated: CD68, Cyr61, VGF, RRAD, PAI1, IL32, ETS2, NOXA, HES1, HSPA1A; Downregulated: ASS1, BTG1, CEBPD, CGA, PFKFB3, LAPTM4B, LTBP1, NR4A3, NR4A1, PDE4D, GDF15, PER1, SMARCA1, FOS
JUN	Upregulated: JUN, CD44, CLIC4, CYR61, GADD45A, MET, DNAJB1, IL18, IL32, SPARC, SERPINE1, PLAU, PLAUR, TNC, VIM; Downregulated: ALDH1A1, CEBPD, CGA, DKK1, MDM2, NR4A1, SLC6A6, SFTPB, F3, FOS, CYBA