Skip to main content
. 2014 Nov 14;9(11):e112723. doi: 10.1371/journal.pone.0112723

Figure 6. mRNA expression in HDSC after antibody-mediated inhibition or siRNA-mediated downregulation of Notch2.

Figure 6

HDSC were differentiated with cAMP/E2P4 for 6 days in the absence or presence of Notch2-blocking antibodies or siRNAs as mentioned in materials and methods. Quantitative real-time PCR analyses showing mRNA expression of the Notch target gene HES1 and the markers of decidualization, IGFBP1 and PRL, upon addition of Notch2 blocking antibodies (A) or Notch2 siRNAs (B). For relative quantification non-stimulated controls (n.c.) were arbitrarily set to 1. Bars indicate mean values ± S.D. of 3 different experiments performed in duplicates. * indicates p≤0.05 compared to n.c.; Significant changes (*, p≤0.05) between Notch2 ab or si-Notch2-treated cells (open bars) and untreated (black bars) cells are indicated by brackets. IgG ctrl, IgG control; si-ntc, si-non targeting control.