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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Chem Biol. 2014 Nov 2;10(12):1061–1065. doi: 10.1038/nchembio.1665

Figure 2. The β2-AR transcriptional response does not require receptor recycling.

Figure 2

(a) Transcriptional activation of PCK1 was measured by qRT-PCR in response to a 5 min pulse of 1 μM isoproterenol and chase with 10 μM alprenolol in cells pre-treated with either 30 μM Dyngo or vehicle (DMSO). Data = average from n = 5. (b) Percent recycled FLAG epitope-tagged β2-AR measured by flow cytometry in cells pre-treated with 0.5 mM Bafilomycin A1 or vehicle (DMSO). Data = mean surface fluorescence from n = 2, 10,000–20,000 cells/condition, each time point in triplicate. (c) PCK1 expression measured by qRT-PCR in cells pre-treated with Bafilomycin A1 and incubated with indicated doses of isoproterenol for 2 hrs. Data are average of n = 2. Baf = Bafilomycin A1, Iso = isoproterenol, Alp = alprenolol, ND = no drug. ** p < 0.005 by unpaired t-test; error bars = ± s.e.m.