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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Magn Reson Med. 2013 Dec;70(6):1728–1738. doi: 10.1002/mrm.24619

FIG. 3.

FIG. 3

Specific, transient labeling of the perisinusoidal ECM. CF is moved into the liver parenchyma 3 h after injection and is cleared from the liver by one week after injection. IF imaging of the liver from a healthy rat sacrificed 1.5 CF injection (e) shows that CF (red) is specifically labeled to the ECM (green). IF of the liver from a healthy rat sacrificed 3 h after injection shows that CF has been internalized into hepatocytes, Kupffer’s cells, and endothelia (f). Cell nuclei are stained with 4’,6-diamidino-2-phenylindole and fluoresce blue. Scale bar = 20 mm. T2*-weighted MRI 1.5 h after injection reveals a spatial pattern of CF-accumulation consistent with the distribution of hepatic sinusoids. CF distribution, observed with MRI, appears diffuse throughout the liver parenchyma at 2 days and 4 days after injection (a–c). CF is no longer detectable in the liver 7 days after injection (d).