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. 2014 Nov 14;9(11):e112720. doi: 10.1371/journal.pone.0112720

Figure 2. DADS inhibits growth and invasion by up-regulating miR-34a in breast cancer cells.

Figure 2

(A) MDA-MB-231 cells were exposed to 0, 25, 50, 100, 200, or 400 µM DADS for 48 h, and the expression of miR-34a was assessed by qRT-PCR. Triplicate assays were performed for each RNA sample, and the relative miRNA levels were normalized to U6 snRNA. *p<0.05 vs. mock, **p<0.01 vs. mock. (B) MDA-MB-231 cells treated with 200 µM DADS and transfected with miR-34a mimics or scramble control miRNA were seeded in 12-well plates at the desired cell concentrations and maintained in medium containing 10% fetal bovine serum. The cells were counted in triplicate at the indicated time points, and the average growth rates were calculated. *p<0.05 vs. mock, **p<0.01 vs. mock. (D) Transwell invasion assay using MDA-MB-231 cells treated with scramble control, miR-34a mimics, mock or miR-34a plus 200 µM DADS and analyzed after 48 h.