Fig. 1.

Kinetic analysis of antigen presentation in mediastinal LNs during primary HKx31 influenza infection. (A) Mediastinal LNs from influenza-infected C57BL/6 mice were treated with collagenase/DNase to form single-cell suspensions. These cells were cultured with a lacZ-inducible hybridoma specific for influenza NP (BWZ-IFA.NP4) and enumerated in duplicate for β-galactosidase-producing cells. Each time point represents the mean of three to five mice. (B) A CD11c⁺ cell is responsible for presentation of D^bNP₃₆₆ after influenza infection. Three days after i.n. infection with HKx31 influenza, mediastinal LNs were pooled (15 mice), and collagenase/DNase was digested to form single cells. These cells either were left undepleted or were depleted of specific cellular subsets by using mAb staining followed by anti-rat Dynabeads. They were then cultured with a lacZ-inducible hybridoma specific for a class I-restricted epitope of influenza NP (BWZ-IFA.NP4). Data show the mean and SD of three separate experiments. Data for CD4 depletion show the mean (bar) and independent values (open circles) of two experiments. *, P < 0.01 or less.