Table 3. Klason lignin concentration and saccharification efficiency of stover from four newly identified bmr loci.
| Glucose Release (mg/g Stover) |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Klason Lignin (%) |
Hydrolysis 4 hr |
Hydrolysis 20 hr |
Hydrolysis 96 hr |
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| Line | lsmean | Lower | Upper | lsmean | Lower | Upper | lsmean | Lower | Upper | lsmean | Lower | Upper |
| BTx623T | 21.2 | 20.5 | 22.0 | 39.9 | 28.9 | 50.8 | 57.7 | 47.1 | 68.2 | 68.8 | 60.5 | 77.0 |
| bmr29 | 18.2 | 16.7 | 19.7 | 39.2 | 30.2 | 48.1 | 49.5 | 38.9 | 60.0 | 57.3 | 49.1 | 65.5 |
| bmr30 | 20.2 | 18.3 | 22.1 | 51.4 | 40.5 | 62.4 | 73.9 | 63.3 | 84.4 | 79.5 | 71.2 | 87.7 |
| bmr31 | 19.7 | 18.1 | 21.2 | 54.7 | 43.8 | 65.7 | 61.1 | 50.6 | 71.7 | 74.3 | 66.1 | 82.5 |
| bmr32-1 | 19.2 | 17.2 | 21.1 | 41.6 | 28.2 | 55.0 | 55.3 | 42.4 | 68.2 | 65.0 | 54.9 | 75.2 |
| bmr32-2 | ND | ND | ND | 43.0 | 29.6 | 56.5 | 58.9 | 46.0 | 71.8 | 59.7 | 49.2 | 70.2 |
| bmr32-3 | 19.2 | 17.5 | 21.0 | 44.5 | 36.4 | 52.6 | 58.0 | 50.2 | 65.8 | 65.5 | 59.1 | 71.8 |
Klason lignin concentration was determined as described in the Materials and Methods section. Glucose yields were obtained after enzymatic saccharification with cellulase of native (unpretreated) stover after 4, 20, and 96 hr. Least squares means (lsmean) for mutant and check lines were generated, and means of all variables were ranked from highest (Upper) to lowest (Lower). Bold text indicates values that were statistically significantly different (P ≤ 0.05) from the values of BTx623T, the line used for mutagenesis. Additional details of the statistical analysis are described in the Materials and Methods section.