Figure 1.

Experimental design. Human umbilical vein endothelial cells (HUVEC) from passage 2 were cultured either in medium containing euglycemic glucose concentration of 5.5 mM or in a hyperglycemic glucose concentration of 16.5 mM. After 24 hr, the euglycemic cultures and a portion of the hyperglycemic cultures were treated with 150 µM CoCl₂ to induce chemical hypoxia. Then, RNA was extracted for gene expression analysis performed with microarrays and qRT-PCR.