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. 2014 Sep 5;4(11):2159–2165. doi: 10.1534/g3.114.013102

Table 1. PCR primers used in validation of gene expression by qRT-PCR.

Gene-Specific Primers Oligonucleotide Primer Sequence 5 to 3 Primer Length (bp) Product Size (bp) Tm (°C)
RPLPO Fw CCATTCTATCATCAACGGGTACAA 24 75 62
Rw TCAGCAAGTGGGAAGGTGTAATC 23 63
GAPDH Fw GCCATCAATGACCCCTTCAT 20 81 58
Rw GCCATGGAATTTGCCAT 17 50
GUSB Fw CTACATCGATGACATCACCGTCAC 24 80 65
Rw TGCCCTTGACAGAGATCTGGTAA 23 63
TFRC Fw GTCGCTGGTCAGTTCGTGATT 21 80 61
Rw AGCAGTTGGCTGTTGTACCTCTC 23 65
ACTB Fw CCCTGGCACCCAGCAC 16 71 58
Rw GCCGATCCACACGGAGTAC 19 62