Table 1. PCR primers used in validation of gene expression by qRT-PCR.
Gene-Specific Primers | Oligonucleotide Primer Sequence 5′ to 3′ | Primer Length (bp) | Product Size (bp) | Tm (°C) |
---|---|---|---|---|
RPLPO Fw | CCATTCTATCATCAACGGGTACAA | 24 | 75 | 62 |
Rw | TCAGCAAGTGGGAAGGTGTAATC | 23 | 63 | |
GAPDH Fw | GCCATCAATGACCCCTTCAT | 20 | 81 | 58 |
Rw | GCCATGGAATTTGCCAT | 17 | 50 | |
GUSB Fw | CTACATCGATGACATCACCGTCAC | 24 | 80 | 65 |
Rw | TGCCCTTGACAGAGATCTGGTAA | 23 | 63 | |
TFRC Fw | GTCGCTGGTCAGTTCGTGATT | 21 | 80 | 61 |
Rw | AGCAGTTGGCTGTTGTACCTCTC | 23 | 65 | |
ACTB Fw | CCCTGGCACCCAGCAC | 16 | 71 | 58 |
Rw | GCCGATCCACACGGAGTAC | 19 | 62 |