Table 2. NormFinder microarray expression stability analysis.
Gene Name | Hypoxia Stability Value | Hyperglycemia/Hypoxia Stability Value | Lower log2-Intensity | Upper log2-Intensity |
---|---|---|---|---|
RPLP0 | 0.008 | 0.004 | 13.05 | 13.31 |
GAPDH | 0.008 | 0.005 | 13.81 | 14.05 |
RPLP2 | 0.009 | 0.005 | 13.03 | 13.18 |
ACTB | 0.010 | 0.005 | 13.28 | 13.56 |
HPRT1 | 0.015 | 0.008 | 9.14 | 9.77 |
GUSB | 0.016 | 0.008 | 8.92 | 9.65 |
TFRC | 0.018 | 0.012 | 10.63 | 11.40 |
ATP5F1 | 0.019 | 0.010 | 7.20 | 7.94 |
B2M | 0.020 | 0.012 | 10.44 | 11.58 |
RPL13A | 0.023 | 0.028 | 9.86 | 11.18 |
Gene expression in the microarray was calculated by log2-intensity values of the genes. NormFinder transforms log to linear scale and ranks the candidate genes according to their expression stability, where a lower value indicates a higher stability in gene expression. The stability of genes was ranked into two groups, hypoxia for different time points (1, 3, 12 hr) and hyperglycemia/hyperglycemia combined with hypoxia for different time points (1, 3, 12 hr).