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. 2014 Sep 5;4(11):2159–2165. doi: 10.1534/g3.114.013102

Table 2. NormFinder microarray expression stability analysis.

Gene Name Hypoxia Stability Value Hyperglycemia/Hypoxia Stability Value Lower log2-Intensity Upper log2-Intensity
RPLP0 0.008 0.004 13.05 13.31
GAPDH 0.008 0.005 13.81 14.05
RPLP2 0.009 0.005 13.03 13.18
ACTB 0.010 0.005 13.28 13.56
HPRT1 0.015 0.008 9.14 9.77
GUSB 0.016 0.008 8.92 9.65
TFRC 0.018 0.012 10.63 11.40
ATP5F1 0.019 0.010 7.20 7.94
B2M 0.020 0.012 10.44 11.58
RPL13A 0.023 0.028 9.86 11.18

Gene expression in the microarray was calculated by log2-intensity values of the genes. NormFinder transforms log to linear scale and ranks the candidate genes according to their expression stability, where a lower value indicates a higher stability in gene expression. The stability of genes was ranked into two groups, hypoxia for different time points (1, 3, 12 hr) and hyperglycemia/hyperglycemia combined with hypoxia for different time points (1, 3, 12 hr).