Table 3. Transcriptome analysis of five selected reference genes from HUVEC cultured under different conditions.

Gene	P Hypoxia 1 hr	FC Hypoxia 1 hr	P Hypoxia 3 hr	FC Hypoxia 3 hr	P Hypoxia 12 hr	FC Hypoxia 12 hr	P High Glucose	FC High Glucose	p value High Glucose + Hypoxia 1 hr	FC High Glucose + Hypoxia 1 hr	p value High Glucose + Hypoxia 3 hr	FC High Glucose + Hypoxia 3 hr	p value High Glucose + Hypoxia 12 hr	FC High Glucose + Hypoxia 12 hr
ACTB	0.870	1.014	0.653	−1.039	0.154	1.132	0.428	1.070	0.320	1.089	0.519	1.056	0.343	1.085
GAPDH	0.962	1.002	0.877	−1.007	0.033	−1.101	0.410	−1.036	0.849	1.008	0.301	−1.046	0.051	−1.092
GUSB	0.993	−1.004	0.937	1.035	0.842	1.090	0.869	1.074	0.919	−1.044	0.926	1.041	0.879	−1.068
RPLP0	0.910	−1.006	0.731	−1.018	0.471	−1.038	0.535	−1.033	0.394	−1.045	0.416	−1.043	0.928	1.005
TFRC	0.836	1.049	0.890	−1.033	0.754	−1.076	0.997	1.001	0.828	−1.052	0.557	−1.147	0.886	1.034

Reference gene expressions from three independent microarray hybridizations were analyzed by Partek genomic suite version 6.6. Gene expressions for all conditions were compared against control sample, which is under euglycaemia and normoxia. Genes showing expression between −1.2-fold and 1.2-fold change and P > 0.05 were considered stable. FC, fold change; ACTB, actin beta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GUSB, glucuronidase beta; RPLP0, ribosomal protein large P0; TFRC, transferrin receptor. The NCBI reference sequences (RefSeq) for: ACTB, NM_001101; GAPDH, NM_002046; GUSB, NM_000181; RPLP0, NM_001002; TFRC, NM_003234.