SoxE and SoxH genes identified in C. gigas and their expression profile. (A) Alignment of HMG domains of CgSoxE, CgSoxH, and homologs from selected vertebrates. (B) Phylogenetic tree of protein sequences of CgSoxE, CgSoxH, and selected genes. (C) Expression profile of CgSoxE and CgSoxH in adult organs of C. gigas with standard deviation as error bars (n = 3). Species names are abbreviated as Cg for Crassostrea gigas, Dm for Drosophila melanogaster, Dr for Danio rerio, Mm for Mus musculus, and Hs for Homo sapiens. Numbers in the tree represent bootstrap values. Accession numbers: CgSoxE EKC31659.1, CgSoxH EKC38002.1, DmSox100B AAF57112.2, MmSry AAI11529.1, HsSry AFG33955.1, HsSox8 AAH31797.1, HsSox9 CAA86598.1, HsSox10 CAG30470.1, and HsSox30 BAA37146.1.