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. 2014 Sep 19;4(11):2247–2257. doi: 10.1534/g3.114.014332

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Copyright © 2014 Ott et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Retrotransposon RNA levels are increased to varying degrees in the various spn-E mutant ovaries. (A) Quantitative real-time RT-PCR for the retrotransposons Het-A and Blood using extracts from mutant ovaries (spn-E^mutant/spn-E^∆125). (B) Quantitative real-time RT-PCR for retrotransposons I Factor, TART, and Roo. Relative expression was calculated in comparison to respective RNA levels obtained from heterozygous siblings for each individual allele. All RNA was normalized to Adh. Error bars represent SD of four experiments using two independent RNA isolates.