Table 1. Overview of the spn-E mutant phenotypes.

Allele Name	SPN-E Protein	Mutation	Affected Domain	D/V Defects	Retrotransposon Expression	Dynein Aggregate Formation	Aub Nuage Localization
2A9-14	+	Thr145Ile	DExH box (I)	Severe	↑↑↑	Yes	Delocalized
7G2-5	+	Thr233Ile	DExH box (I/II)	Severe	↑↑↑	Yes	Delocalized
155-55	+	Glu239Lys	DExH box (II)	Mid	↑↑↑	Yes	Delocalized
23-17	+	His241Gln	DExH box (II)	Mild	↑	No	Partial
8D4-11	+	Ser435Phe	DExH box (V)	Severe	↑↑↑	Yes	Delocalized
9A2-17	+	Pro508Leu	None	Mild	↑↑	No	Partial
4-48	+	Glu717Lys	None	Mild	↑	No	Nuage
66-21	+	His1422Leu	Zn finger	Mild	↑	No	Nuage
100-37	—	G→A bp 488 splice site	Truncated at aa 151	Mid	↑↑↑	Yes	Delocalized
114-33	—	Arg918stop	Truncated at aa 918	Severe	↑↑↑	Yes	Delocalized
9A9-18	—	Trp632stop	Truncated at aa 632	Severe	↑↑↑	Yes	Delocalized
4E2-14	—	Arg695stop	Truncated at aa 695	Severe	↑↑↑	Yes	Delocalized
616	—	Arg1081stop	Truncated at aa 1081	Mid	↑↑↑	Yes	Delocalized
653	—	Tyr636stop	Truncated at aa 636	Severe	↑↑↑	Yes	Delocalized

+ or − for SPN-E protein indicates: (+) SPN-E protein detected; (−) SPN-E protein not detected on Western blot analysis. Number in parentheses next to DExH box indicates the DExH box motif affected. Severe D/V defects indicate mostly collapsed eggs laid by mutant females; mid indicates the majority of eggs laid were collapsed; however, a small percent have wild-type or fused dorsal appendages; mild indicates the majority of eggs laid were wild-type. ↑↑↑ indicates all retrotransposons were upregulated in the mutant ovaries; ↑↑ indicates that at least two retrotransposons tested were upregulated; ↑ indicates that one or no retrotransposons was upregulated in the mutant ovaries. bp numbering for 100-37 according to Ensemble Genome Browser. ND, not determined.