Table 1. Analysis of CUP1 gene tandem arrays in 14 yeast strains of Saccharomyces cerevisiae.
| Strain | Type of Repeat (size in kb) | Size of EcoRI fragment, kba | CUP1 Copy No. Southern | CUP1 Copy No. (Deep Sequencing)b | [Cu2+] Inhibitory Concentration, mM |
|---|---|---|---|---|---|
| S288c | 1 (2.0) | 30.8 | 14 | ND | 2 |
| W303-1A | 1 (2.0) | 31.3 | 14 | ND | 1.8 |
| YJM189 | 2 (1.8) | 23.3 | 11 | 6 | 0.8 |
| YJM972 | 2 (1.8) | 18.7 | 8 | 10 | 1 |
| YJM996 | 2 (1.8) | 25.4 | 12 | 12 | 1.4 |
| YJM789 | 3 (1.2) | 11.6 | 7 | ND | 0.4 |
| YJM693 | 3 (1.2) | 19.3 | 13 | 15 | 1.8 |
| YJM1549 | 3 (1.2) | 24.4 | 18 | 18 | 1.0 |
| YJM271 | 4 (1.9) | 20.7 | 9 | 8 | 0.3 |
| YJM1307 | 4 (1.9) | 15.5 | 6 | 4 | 0.3 |
| YJM456 | 5 (1.6) | 19.8 | 10 | 5 | 1.2 |
| YJM969c | 5 (1.6) | 8.5 | 3 | 4 | 0.2 |
| YJM978 | 5 (1.6) | 6.5 | 2 | 2 | 0.1 |
| DTY3 | No repeat | 5.2d | 1 | NR | <0.1 |
ND, not determined. NR, not relevant.
Average size of CUP1-containing EcoRI fragment based on two to three independent experiments.
Estimate of CUP1 copy number based on coverage in deep-sequencing analysis (P. K. Strope, D. A. Skelly, S. G. Kozmin, G. Mahadevan, E. A. Stone, P. M. Magwene, F. S. Dietrich, and J. H. McCusker, personal communication).
This strain had a mixture of Type 3 and Type 5 repeats by deep sequencing (P. K. Strope, D. A. Skelly, S. G. Kozmin, G. Mahadevan, E. A. Stone, P. M. Magwene, F. S. Dietrich, and J. H. McCusker, personal communication), but only Type 5 repeats by polymerase chain reaction analysis.
This size is based on our sequencing results and on Southern analysis of a single-copy CUP1 strain (Fogel et al. 1983).