Fig. 1.

Impact of cholesterol depletion on Kir2.1–PIP2 interactions.

A. Representative Kir2.1 current traces before (red) and after (green) dialyzing neomycin into the cell through a patch pipette. The upper family of traces was recorded in a cell in normal cholesterol conditions and the lower family of traces recorded from a cell depleted of cholesterol by pre-exposure to 5 mM MβCD for 1 h. B: The time courses of Kir2.1 rundown in response (1–100 μM) neomycin. C: The time courses of Kir2.1 rundown in response 10 μM neomycin in control and cholesterol depleted cells. D: The time courses of Kir2.1 rundown in response anti-PIP2 antibodies in control and cholesterol depleted cells (all data show means + SEM, n = 5–10 cells per condition).