Table 1.
Overview comparison of next-generation sequencing techniques.
| Platform | Method | Read length (bp) | Throughput |
|---|---|---|---|
| Roche 454 | Pyrosequencing | 400 | 400 Mb/run |
| Illumina/Solexa HiSeq | Reversible terminator chemistry | 2 × 100 | 600 Gb/run |
| ABI SOLiD | Ligation | 2 × 60 | 15 Gb/day |
| HelicosBiotechnology | Reversible terminator chemistry | 25–55 | 28 Gb/run |
| Roche 454 — GS Junior | Pyrosequencing | 400 | 50 Mb/run |
| Illumina/Solexa MiSeq | Reversible terminator chemistry | 2 × 150 | 1.0–1.4 Gb/run |
| ABI Iontorrent | H + ion selective transistor | – | 320 Mb/run |