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. 2004 May 24;101(23):8699–8704. doi: 10.1073/pnas.0401142101

Fig. 2.

Fig. 2.

BubR1 kinase activity is required for mitotic checkpoint signaling. (A) Total cellular lysates of T98G cells transfected with mock or BubR1 siRNA plasmid in combination with either empty vector or myc-tagged siRNA-resistant BubR1 mutants were immunoblotted for BubR1 or the myc epitope tag. KD, K795A. (B) T98G cells were transfected, treated, and analyzed as in Fig. 1D, with the addition of the various siRNA-resistant BubR1 alleles during transfection. Percentage BrdUrd positivity in the mock samples without colcemid was set to 100. (C) Stills of live cell microscopy of BubR1 siRNA HeLa cells transfected with pH2B-EYFP and the indicated siRNA-resistant BubR1 alleles. Forty-eight hours posttransfection, images of H2B-EYFP-positive cells were taken at 2-min intervals. Arrows indicate unaligned chromosomes during anaphase.