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. 2004 May 24;101(23):8699–8704. doi: 10.1073/pnas.0401142101

Fig. 3.

Fig. 3.

Mitosis with reduced BubR1 or Mad2 causes acute chromosome loss. (A) DNA content profiles of HeLa cells transfected with the indicated siRNA plasmids along with pBabe-Puro for 24 hr and grown in puromycin-containing medium for an additional 48 hr. Nontransfected cells were removed 14 hr before analysis. (B) Distribution of the amount of chromosomes within a G1 population of YCA-2A3 cells transfected with mock, BubR1 siRNA, or Mad2 siRNA. Images are Z-stack projections displaying all EYFP-CENP-A-containing centromeres in one plane of a transfected (H2B-ECFP-positive) cell. Number in parentheses indicates number of chromosomes.