Figure 3.

SDS-PAGE analysis of the proteins secreted into the liquid medium with EFB as the sole carbon source. The medium was taken out and concentrated before mixing with SDS-PAGE loading dye. The samples were heated at 100°C for 3 min then loaded onto SDS-PAGE gel. The protein bands were visualized by Coomassie Blue staining. The numbers listed on the left side of the gel are the numbers of proteins listed in Table 1. The upper panel of the gel shows the cultivation time of Neurospora crassa S1.