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. 2004 May 25;101(23):8774–8779. doi: 10.1073/pnas.0402795101

Fig. 3.

Fig. 3.

Involvement of the RhoA signaling pathway in the BDNF inhibition of migration. Schwann cells (A and B), and Cos-7 cells (E and F) transfected with p75NTR, were pretreated with C3 exoenzyme (A and E) or Y-27632 (B and F). After incubation with BDNF, migration was assayed by using collagen-coated Boyden chambers (A, B, E, and F). After stimulation with BDNF (0–45 min), RhoA activity was measured by pull-down assay using GST-mDia1-Rho-binding domain in the lysates from Schwann cells (C) and Cos-7 cells (G) transfected with RhoA and p75NTR. The total RhoA in the cell lysates is also shown (C and G). RhoA activity was measured in Schwann cells (D) transfected with control or p75NTR siRNA, and Cos-7 cells (H) were transfected with RhoA ± p75NTR.