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. 2004 May 25;101(23):8774–8779. doi: 10.1073/pnas.0402795101

Fig. 5.

Fig. 5.

Migration is regulated by Src kinase-dependent Vav2 activation. (A) Schwann cells were transfected with control or Vav2 siRNA. (D) Cos-7 cells were transfected with or without Vav2ΔCat and p75NTR. After incubation with BDNF, migration was assayed by using collagen-coated Boyden chambers (A and D). To confirm the effects of siRNAs, lysates from transfected cells were immunoblotted with anti-Vav2, RhoA, or β-actin antibody (A). Active Vav2 was detected by the pull-down assay using GST-RhoAG15A from the lysates of Schwann cells (B), and Cos-7 cells (E) were transfected with Vav2 and p75NTR. The total Vav2 in the cell lysates is shown also (B and E). Schwann cells were transfected with a control or the Vav2 siRNA (C); Cos-7 cells were transfected with or without Vav2ΔCat, RhoA, and p75NTR (F); and RhoA activity was then measured.