Fig. 5.

Precocious atoh7 expression results in predominant RGC differentiation in the ventral retina. (A–H) Anti-HuC/D immunohistochemistry performed on frontal sections of control (A–D) and rx2::atoh7 (E–H) hatchlings, scale bar 20 μm. Notably a broader HuC/D positive area can be observed in the ventral retina of rx2::atoh7 fish. (I–L) ventral views of whole mount in situ hybridizations performed with probes for brn3c (I, control and J, rx2::atoh7) and islet2 (K, control and L, rx2::atoh7). Notably an extended expression of brn3c and islet2 along the optic fissure region can be observed in rx2::atoh7 hatchlings.