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Determination of eNOS protein expression by Western blot of aortic protein extracts of hypercholesterolemic mice with the use of eNOS and γ-tubulin antibodies. Films were quantified by densitometry and results were expressed as mean ± SD of arbitrary units. (Insets) Representative blots of each group. *, P < 0.05 vs. regular diet; §, P < 0.01 vs. respective lane in no exercise; **, P < 0.01 vs. diet plus antioxidants plus l-arginine no exercise and diet plus antioxidants and exercise.
