. 2014 Autumn;13(4):1263–1277.

Table 2.

Protective effects of different pistachios extracts against cumene hydroperoxide and glyoxal induced ROS formation.

ROS formation			Addition
60 min	30 min	15 min	Addition
193 ± 9	158 ± 7	114 ± 9	control
531 ± 12^a	291 ± 9^a	225 ± 8^a	+cumene hydroperoxide(120 µM)
241 ± 12^b	182 ± 8^b	127 ± 5^b	+butylatedhydroxytoluene (50 μM)
417 ± 14^b	246 ± 8^b	174 ± 5^b	+pistachios ethyl acetate extract (150 µg/mL)
305 ± 11^b	183 ± 6^b	146 ± 4^b	+pistachios methanolic extract (150 µg/mL)
361 ± 10^b	213 ± 8^b	167 ± 5^b	+pistachios water extract (150 µg/mL)
422 ± 11^b	250 ± 8^b	181 ± 9^b	+α-tochoferol(100 μM)
385 ± 9^b	225 ± 8^b	172 ± 7^b	+gallic acid (100 μM)
368 ± 9^b	217 ± 8^b	169 ± 5^b	+catechin (5 mM)
449 ± 9^a	256 ± 9^a	194 ± 8^a	+glyoxal (5 mM)
228 ± 7^b	163 ± 5^b	117 ± 8^b	+butylatedhydroxytoluene (50 μM)
339 ± 7^b	204 ± 8^b	152 ± 4^b	+pistachios ethyl acetate extract (150 µg/mL)
270 ± 7^b	177 ± 6^b	126 ± 5^b	+pistachios methanolic extract (150 µg/mL)
293 ± 8^b	193 ± 10^b	154 ± 9^b	+pistachios water extract (150 µg/mL)
342 ± 8^b	210 ± 8^b	160 ± 5^b	+α-tochoferol(100 μM)
299 ± 8^b	199 ± 7^b	153 ± 5^b	+gallic acid (100 μM)
295 ± 8^b	197 ± 7^b	158 ± 5^b	+catechin (5 mM)

Hepatocytes (10⁶cells/mL) were incubated in Krebs–Henseleit buffer pH 7.4 at 37^◦C for 1.0 h following the addition of EC50_2h of cumene hydroperoxide and glyoxal. DCF formation was expressed as fluorescent intensity units (Shen et al., 1996).

Values are expressed as mean±SD of three separate experiments (n=5).

Significant difference in comparison with control hepatocytes (P < 0.05).

Significant difference in comparison with cumene hydroperoxide or glyoxal treated hepatocytes (P < 0.05).