. 2014 Autumn;13(4):1263–1277.

Table 6.

Protective effects of different pistachios extracts against cumene hydroperoxide and glyoxal induced Lysosomal membrane leakiness in rat hepatocytes

Percent of acridine orange redistribution			Addition
60 min	30 min	15 min	Addition
5 ± 1^b	3 ± 1^b	2 ± 1^b	control
28 ± 2^b	17 ± 2^b	11 ± 2^b	+cumene hydroperoxide (120 µM)
6 ± 1^b	3 ± 1^b	3 ± 1^b	+chloroquine (100 µM)
20 ± 2^b	12 ± 2^b	5 ± 1^b	+pistachios ethyl acetate extract (150 µg/mL)
14 ± 2^b	9 ± 1^b	3 ± 1^b	+pistachios methanolic extract (150 µg/mL)
17 ± 2^b	11 ± 2^b	5 ± 1^b	+pistachios water extract (150 µg/mL)
19 ± 3^b	13 ± 2^b	6 ± 1^b	+α-tochoferol(100 μM)
16 ± 1^b	9 ± 1^b	3 ± 1^b	+gallic acid (100 μM)
17 ± 1^b	10 ± 2 ^b	4 ± 1^b	+catechin (5 mM)
21 ± 2^b	13 ± 2^b	8 ± 1^b	+glyoxal (5 mM)
8 ± 1^b	3 ± 1^b	2 ± 1^b	+chloroquine (100 µM)
15 ± 2^b	8 ± 1^b	6 ± 1 ^b	+pistachios ethyl acetate extract (150 µg/mL)
9 ± 1^b	6 ±1^b	3 ± 1^b	+pistachios methanolic extract (150 µg/mL)
12 ± 2^b	8 ± 1^b	7 ± 1 ^b	+pistachios water extract (150 µg/mL)
15 ± 2^b	9 ± 1^b	8 ± 1 ^b	+α-tochoferol(100 μM)
9 ± 1^b	6 ± 1^b	3 ± 1^b	+gallic acid (100 μM)
13 ± 2^b	9 ± 1^b	7 ± 1^b	+catechin (5 mM)

Hepatocytes (10⁶cells/mL) were incubated in Krebs–Henseleit buffer pH 7.4 at 37^◦C for 1.0 h following the addition of EC50_2h of cumene hydroperoxide and glyoxal. Lysosomal membrane damage was determined as intensity unit of diffuse cytosolic green fluorescence induced by acridine orange following the release from lysosomes. Our data were shown as the percentage of lysosomal membrane leakiness in all treated (test) hepatocyte groups (Pourahmad et al., 2001)

Values are expressed as mean±SD of three separate experiments (n=5).

Significant difference in comparison with control hepatocytes (P < 0.05).

Significant difference in comparison with cumene hydroperoxide or glyoxal treated hepatocytes (P < 0.05).