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. 2014 Autumn;13(4):1335–1345.

Table 1.

Cytotoxic effects of different concentrations of F. angulata Boiss. extracts on AGS cells. Each value is represented by the mean ± S.E.M. of three independent experiments (n = 3) of three replicates (P < 0.05 vs. control group).

A) Trypan blue assay
Concentration μg/mL
Plant part
tested
20 40 60 80 100 120 140 160
Leaves 24 h 13.0±2.08 25.0±1.52 32.0±3.05 41.0±2.64 51.0±1.52 70.0±2.08 85.0±1.52 100.0±0
48 h 22.0±2.51 39.67±2.18 54.0±1.52 66.0±2.30 82.0±3.05 100.0±0 100.0±0 100.0±0
Flowers 24 h 10.0±2.513 14.67±2.90 24.0±2.52 29.33±1.20 40.0±1.15 54.0±3.05 76.0±1.73 88.0±1.53
48 h 15.0±2.08 30.0±2.31 46.0±1.53 62.0±2.52 71.0±1.15 92.0±2.0 100.0±0 100.0±0
S.E.M. = Standard error of the mean.
B) Neutral red assay
Concentration μg/mL
Plant part
tested
20 40 60 80 100 120 140 160
Leaves 24 h 3.85±0.63 18.05±1.25 38.05±1.47 51.67±0.90 75.62±1.79 84.91±0.41 100.0±0 100.0±0
48 h 19.45±0.26 50.87±0.58 77.80±0.70 100.0±0 100.0±0 100.0±0 100.0±0 100.0±0
Flowers 24 h 3.84±1.29 8.77±2.32 33.04±0.93 38.73±0.91 49.93±0.76 69.27±1.19 86.68±0.79 100.0±0
48 h 14.75±1.79 37.06±1.48 67.05±0.54 85.62±0.92 100.0±0 100.0±0 100.0±0 100.0±0

S.E.M. = Standard error of the mean.