. 2004 Jun 3;23(12):2381–2391. doi: 10.1038/sj.emboj.7600241

Table 2.

Comparison of Prp19/CDC5 complexes

14S CDC5/hPrp19 complex (this study)^a	Human CDC5 complex (Ajuh et al, 2000)^b	Prp19 complex (Chen et al, 2002)^c	Prp19 complex (Ohi et al, 2002)^d
hPrp19	hPrp19/CCAP3^e	Prp19	Prp19
CDC5	CDC5L^e	Ntc85/Cef1/Cdc5	Cef1/Cdc5
PRL1	PRL1/hPrp46^e		Cwc1/Prp46
AD-002	AD-002/CCAP2
HSP73	HSP73/CCAP1^e
SPF27	SPF27^e	Ntc25/Snt309^f	Snt309^f
β-catenin like
	CCAP4
	CCAP5
	CCAP6^e
	CCAP7
	CCAP8
	ASF/SF2
	PSF
	SRm160
	SC35
	NF45
	TOPIIa
	U1A
	hnRNPG
	PP2c (WIP1)
	DDP kinase
	PP1-α
	Sm proteins		Sm proteins
	U2-specific proteins		U2-specific proteins
			U5-specific proteins
		Ntc77/Clf1/Syf3	Clf1/Syf3
		Ntc90/Syf1	Syf1
		Ntc30/Isy1	Isy1
		Ntc31/Syf2	Syf2
		Ntc20	Ntc20
			Slu7
			Ecm2
			Spp2
			Cwc2, Cwc14, Cwc15
			Cwc16 Cwc21, Cwc22
			Cwc23, Cwc24, Cwc25 Cwc26, Cwc27
			Prp17, Prp22, Prp45
^a Complexes were immunoaffinity purified from HeLa nuclear extract with anti-CDC5 antibodies at 200 mM salt and further purified by glycerol gradient centrifugation.
^b Complexes were purified from HeLa nuclear extracts either by immunoaffinity chromatography with anti-CDC5 antibodies at 150 mM salt or by tandem chromatographic steps involving gel filtration and ion exchange chromatography (Ajuh et al, 2000).
^c Complexes were isolated from S. cerevisiae total cell extracts at 60 mM salt by tagging the Prp19 protein with an HA-tag and precipitating with anti-HA antibodies (Tarn et al, 1994).
^d Complexes were isolated from S. cerevisiae total cell extracts using the TAP method (150 mM salt) after tagging the Prp19 protein (Ohi et al, 2002).
^e Stably associated core protein in the CDC5 protein complex (Ajuh et al, 2000).
^f Snt309 is a functional counterpart of S. pombe Cwf7 that is homologous to SPF27 (Ohi and Gould, 2002).