Figure 3. Behavioural responsiveness following cocaine administration in honey bees.

(A) Proportion of bees responding to 10% sucrose following treatment with 0 or 10 µg of volatilised cocaine (error bars represents standard error and letters denote statistically different groups). There was a significant increase in sucrose responsiveness in bees treated with 10 µg cocaine relative to control (χ² = 6.1013, df = 1, p = 0.0135). (B) Proportion of bees responding to 10% sucrose following treatment with 0, 5, 10, 20, or 50 µg of volatilised cocaine. There was a dose-dependent relationship between cocaine dose and sucrose responsiveness (χ² = 14.089, df = 4, p = 0.0070). (C) Shock responsiveness of bees following cocaine administration. Curves are based on weibull distributions of shock responsiveness for each group. Comparisons are based on estimates of EV₅₀ for 40 bees per group (F_4,40 = 5.4, p = 0.0015). Pairwise comparisons found that the 50 µg group was different from all other groups, while the remaining cocaine treated groups were different from controls.