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Effect of ChK on Akt phosphorylation in H2009 human carcinoma cells. Cells were treated with vehicle (DMSO) or 5 μM ChK for 4, 12, or 24 h and extracted for Western blot analysis of phospho-Akt at ser473 or total Akt. Quantification is shown in graph to the right. *Statistically significant (p<0.05) compared to control.
