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FISH micrographs of cross sections through the posterior trophosome region treated with an hierarchical probe set. 4′,6-diamidino-2-phenylindole (DAPI) was used as a counter stain (blue). A. Probe Gam42a in Fluos (green) targeting Gammaproteobacteria. B. Probe Scon −467 in Cy5 (red) specific for the S. contortum endosymbionts. C. Overlay. Bc, bacteriocyte; ep, epidermis; ml, muscle layer; py, pyriform gland.
