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. 2004 May 20;23(12):2451–2458. doi: 10.1038/sj.emboj.7600246

Figure 4.

Figure 4

Analysis of the expression pattern of h3G and h3F mRNA by semiquantitative RT–PCR. (A) This experiment utilized a commercially obtained pre-normalized human multiple tissue cDNA panel. Positive controls represent non-normalized cDNA preparations derived from EBV-transformed B cells (lane 9) or human K562 cells (lane 10). Neg: no added cDNA. (B) Similar to panel A, except that a pre-normalized panel of human lymphoid tissue cDNAs was used. (C) This RT–PCR experiment utilized total RNA preparations derived from human H9, CEM or CEM-SS cells or mouse T cells. The GAPDH gene was used as an internal control. Neg: no added mRNA.