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. 2014 Aug 7;204(1):74–80. doi: 10.1111/nph.12986

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© 2014 The Authors New Phytologist © 2014 New Phytologist Trust

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Transient expression of split yellow fluorescent protein (YFP) constructs in rice protoplasts. (a) FM4-64FX dye image; red fluorescence reflects the position of the plasma membrane. (b) YFP fluorescence images: protoplasts were transfected with OsNRT2.3a-cEYFP (T2.3a) and nEYFP-OsNAR2.1 (R). (c) Rice protoplasts expressing EYFP (yellow) with FM4-64FX (red) fluorescence. (d) Rice protoplasts in bright field without exciting light. Column 1, protoplasts expressing YFP co-transfected with PSAT1-nEYFP-C1 and PSAT1-cEYFP-N1 as a control (eYFP). Column 2, protoplasts transfected with OsNRT2.3a-cEYFP (T2.3a) and nEYFP-OsNAR2.1 (R) with FM4-64 dye. Column 3, protoplasts transfected with OsNRT2.3a-cEYFP (T2.3a) and nEYFP-OsNAR2.1 R100G (R-R100G) with FM4-64FX dye. Column 4, protoplasts transfected with OsNRT2.3a-cEYFP (T2.3a) and nEYFP-OsNAR2.1 D109N (R-D109N) with FM4-64FX dye. Bars, 10 μm.