Figure 3.

G2+ NK cells mediate in vivo missing-self rejection of BM cellular targets without D^k in BMT chimeric mice. BMT recipients were i.v. injected with a 1:1 mixture of BM cells from Tg-D^k (CTV-labeled) and Non-Tg (CFSE-labeled) mice. After 20 h, spleen and liver leukocytes were collected and analyzed by flow cytometry. (A) Representative dot plots show residual labeled target cells in spleen and liver for the indicated BMT recipients. (B) The results from (A) are summarized in the graph with bars representing the mean (±SEM) ratio of the percentage of residual non-Tg / Tg-D^k BM target cells from the indicated BMT mice. (C) Rejection of Non-Tg BM cells is shown as a ratio of remaining Non-Tg to Tg-D^k BM cells in control Ig, total NK cells, G2+ NK cell (4D11 and AT8) depleted, and Ly49I+ NK cell (1F8) depleted BMT (T-T) mice. Data are shown as the mean ±SEM. **p < 0.01; ***p < 0.001 compared with T-T. Data are representative of two independent experiments. Each individual experiment contains four mice.