Assessing rv1411c, rv1911c, rv2270 and rv3763 genes presence. (A) Amplification of the rpoB gene from gDNA isolated from the species and strains included in this study. MWM: molecular weight marker (1 kb); 1. M. tuberculosis H37Rv; 2. M. tuberculosis H37Ra; 3. M. bovis; 4. M. bovis BCG.
(B) PCR amplification of gDNA isolated from the mycobacterial species and strains evaluated here. MWM: molecular weight marker (1 kb); Lanes 1-4 show rv1411 gene presence in: (1) M. tuberculosis H37Rv, (2) M. tuberculosis H37Ra, (3) M. bovis, (4) and M. bovis BCG; Lanes 5-8 show rv1911 gene presence in: (5) M. tuberculosis H37Rv, (6) M. tuberculosis H37Ra, (7) M. bovis and (8) M. bovis BCG. Lanes 9-12 show rv2270 gene presence in: (9) M. tuberculosis H37Rv, (10) M. tuberculosis H37Ra, (11) M. bovis and (12) M. bovis BCG; Lanes 13-16 show rv3763 gene presence in: (13) M. tuberculosis H37Rv, (14) M. tuberculosis H37Ra, (15) M. bovis and (16) M. bovis BCG. NC. Negative PCR control.