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. 2014 Nov 7;15(1):964. doi: 10.1186/1471-2164-15-964

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© Rao et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of lignin-related gene expression in the early stage seeds and stems. qRT-PCR was performed to verify the expression patterns of PAL, 4CL, CCR, CAD, C4H and CCoAOMT in the light and dark pod tissues, seeds at 6, 8 and 10 weeks and in stems. Expression values were normalized to expression levels of actin, and the expression value in 6 W dark was set to 1. The results are the means of duplicate measurements of two biological replicates, and p-values for difference in expression between 8 week seed tissue and 6 week dark pod tissues were less than 0.01 (Student’s t-test) for all transcripts tested.