Fig. 3.
Ultrastructural detection of TG2 in HEK293TG cells shows distinct organelle localization of the enzyme. a Electron micrograph of a cytoplasmic region containing two mitochondria (m) exhibiting negative or faint staining and a lysosome (Ly) that is clearly labeled. b Mitochondrion displaying gold granules associated with both the outer membrane and the matrix inside the organelle. c Numerous gold particles reveal that TG2 is localized to the endoplasmic reticulum (ER) compartment. d Double-immunolabeling with specific antibodies against TG2 (arrows) and calreticulin (small gold granules) shows a positive reaction on the ER cisternae. Bars 0.3 μm