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. Author manuscript; available in PMC: 2015 May 14.
Published in final edited form as: Adv Funct Mater. 2014 Jan 13;24(18):2648–2657. doi: 10.1002/adfm.201303891

Figure 7.

Figure 7

Fluorescent images showing encapsulated live (green) and dead (red) cells at different time points after BES. Cells encapsulated in 1.5% food-grade alginate are shown a) one day, b) 4 days, and c) 7 days post-encapsulation. Cells encapsulated at d) 1 × 106 cells ml-1 (Low), e) 5 × 106 cells ml-1 (Medium), and f) 10 × 106 cells ml-1 (High). Images were taken 7 days post-encapsulation. Green fluorescence is emitted from intracellular esterase-converted calcein in live cells, whereas red fluorescence is emitted from ethidium homodimer present in the nuclei of dead cells. BES conditions were 0.4 mm inner diameter nozzle, 10 ml h-1 alginate solution flow rate, ∼7 kV applied voltage. Scale bar 500 μm.