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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: Cancer Res. 2014 Oct 1;74(22):6661–6670. doi: 10.1158/0008-5472.CAN-13-3742

Figure 2. RABL6A is required for PNET cell proliferation.

Figure 2

A, Schematic of RABL6 isoforms and RABL6A mRNA regions targeted by KD1 and KD2 shRNAs. B, western blot showing effective RABL6A knockdown in BON-1 cells expressing KD1 and KD2 shRNAs compared to vector control (CON). Graph shows reduced cell numbers following RABL6A knockdown relative to CON cells (*, p<0.05). C, flow cytometric analyses of DNA content show RABL6A depletion causes cell cycle arrest predominantly in G1 phase along with a moderate G2/M arrest. D, infected BON-1 cells were kept under antibiotic selection to maintain shRNA expression and cells counted at the indicated days. A representative growth curve from more than three independent experiments shows RABL6A loss causes sustained inhibition of proliferation.