Figure 4. Characterization of SNF5-inducible MRT cell lines.

Cells were induced with doxycycline (DOX) at Time 0 and samples were harvested at 24 hours after induction. Total cellular proteins (30 μg) were separated on a 4% to 12% Bis-Tris polyacrylamide gel, transferred to PVDF membranes and probed with indicated antibody. KU served as the loading control. UN, untreated control