Figure 5. SWI/SNF complex composition changes after SNF5 re-expression.
(A) The relative abundance of SWI/SNF complex components in the absence (untreated) and presence (Dox) of SNF5 in the TTC642 cell line was determined by label-free quantitative mass spectrometry. The identity of BCL7B could not unambiguously be determined versus BCL7C due to shared peptides. (B) The indicated cell lines (A–C) were induced with doxycycline, and the samples were immunoprecipitated with either rabbit IgG or anti-BRG1. IP samples were then separated on a 4% to 12% Bis-Tris polyacrylamide gel, transferred to PVDF membranes and probed with the indicated antibodies.
