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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Cancer Res. 2014 Jul 9;12(11):1574–1585. doi: 10.1158/1541-7786.MCR-14-0005

Figure 6. Reduction of SWI/SNF complex protein levels after inhibition of SNF5 in normal human fibroblasts and the effects of MG132.

Figure 6

(A) SNF5 knockdown cells were generated by infecting normal human fibroblasts (NHF-1) with lentivirus expressing 2 different shRNAs against SNF5 (TRCN 39585 and TRCN 39587) or a non-targeting shRNA control (pLKO.1) and immediate selection with puromycin. After 72 hrs. on selective medium, total cellular protein (30 μg), was separated on a 4% to 12% Bis-Tris polyacrylamide gel, transferred to PVDF membranes and probed with the indicated antibodies. Ku served as the loading control. (B) NHF-1 hTERT cells were infected with indicated lentivirus and placed in selective media for 3 days. Six hours before harvesting for protein isolation, half of the samples were treated with the proteasome inhibitor MG132. After protein isolation, levels for the indicated proteins were detected by SDS-PAGE and Western blotting. c-FOS served as a positive control for proteasome inhibition while Ran, unaffected by MG132 treatment, served as a negative control. Ku served as the loading control.