The TTC642 pINDUCER20-fSNF5-HA, G401 pINDUCER20-fSNF5-HA and TTC549 pINDUCER20-fSNF5-HA cell lines, were treated with DMSO, 1μg/ml doxycycline or 10uM MG132 for 6 hrs. After protein isolation, levels for the indicated proteins were detected by SDS-PAGE and Western blotting as previously described (Chai et al., 2005a). c-FOS served as a positive control for proteasome inhibition while Ran, unaffected by MG132 treatment, served as a negative control. Ku served as the loading control.