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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Cancer Res. 2014 Jul 9;12(11):1663–1676. doi: 10.1158/1541-7786.MCR-14-0136

Figure 2. Cyclosporin A promotes endothelial cell proliferation and migration in vitro.

Figure 2

A, proliferation of primary lung endothelial cells (LuEC) with CsA or vehicle, n=3 per group. B, representative images of BrdU uptake by LuEC treated with 0.25µM CsA, 5ng/mL EGF or vehicle for 48 hours. Quantification of BrdU+ cells is shown on the right. C, quantification of TUNEL+ LuEC after treatment with vehicle or CsA for 72 hours or doxorubicin (Dox) for 24 hours, n=5 per group. D, representative images of LuEC migration through a transwell filter in response to vehicle or 0.25µM CsA treatment. 100ng/mL FGF is used as a positive control. Scale bar, 100µm. Quantification of cells per high-powered field (hpf) from 8–10 randomly taken images is shown on the bottom. Representative results of three independent experiments is shown. Data is presented as mean ± SD, statistical analysis was performed by a student t-test, * P<0.05.